A bacteriological laboratory is examining canned vegetables that have caused botulism in several people. What cultivation conditions play a leading role in the detection of the causative agents in the test material?

Answer options

1. The temperature of the nutrient medium must not exceed 35°C
2. No oxygen
3. The nutrient medium contains vitamins and ammo acids
4. Antibiotics are added into the nutrient medium to inhibit gram- negative microflora
5. Alkaline reaction of the nutrient medium